[English][Japanese]

Welcome to Shosuke Kojo's Home Page

Name

Shosuke Kojo

Title

Professor

Year of Birth

1948

Place of Birth

Osaka, JAPAN


Research Program

Radical Reactions in Biology


Radical reactions caused by reactive oxygen species (ROS) in the cell have received growing attentions in relation to ageing, diseases such as cancer, atherosclerosis and diabetes, and toxic reactions caused by environmental pollutants. We make a chemical approach to the evaluation of oxidative stress using typical pathological conditions in such as chemically induced hepatitis and diabetes. We found that liver vitamin C concentration is the most sensitive indicator of oxidative stress.


We are also interested in the pathogenesis of atherosclerosis. Our study showed following points.

1.           Radical reaction of LDL (low-density lipoprotein) causes fragmentation and conjugation of apolipoprotein B-100 (apoB), which is a protein component of LDL.

2.           The reactivity of apoB toward radicals is even comparable to vitamin E, a typical antioxidant.

3.           Then products of oxidation of apoB, namely fragmented and aggregated apoB are present in normal human serum. The sum of these oxidation products as determined by Western blot analysis correlated positively with conventional clinical indices such as LDL cholesterol, triglyceride, age, and IMT (intima-media thickness of the carotid artery). These oxidation products as named B-ox negatively correlates with HDL cholesterol and plasma vitamin C.

4.           The plasma ceramide level determined by LC-MS/MS correlated positively with plasma cholesterol. Ceramide is a biologically active lipid causing apoptosis in variety of cells. We are interested in metabolic relationship between these lipids.

 

Origin of homochirality on the prebiotic earth


The predominance of L-aminoacids over D-amino acids in the biosphere has been a long mystery since Pasteur found chirality in 19th century. Despite much effort devoted to elucidating how and why L-amino acids were preferentially selected in the prebiotic earth, no clear solution has been obtained. It has been suggested that L-amino acids originated from cometary and asteroidal materials. However, we showed experimentally that it is the nature of a mixture of racemic amino acids to produce a spontaneous high enantiomeric excess. This study was published in Chem Comm. in 2004 and the paper was selected as a hot paper..



Publications List

 

Review

1. Vitamin C, Basic Metabolism and Its Function as an Index of Oxidative Stress. S. Kojo, Curr. Med. Chem., 11, 1041-1064 (2004).

 

Regular articles

 

1. Involvement of 101F6, a homologue of cytochrome b561, in the reduction of ferric ions.  A. Mizutani, R. Sanuki, K. Kakimoto, S. Kojo, and S. Taketani, J. Biochem. (Tokyo), in press (2007).

2. The effect of dietary cholesterol and high fat on ceramide concentration in rat tissues. I. Ichi, K. Nakahara, K. Kiso, and S. Kojo, Nutrition, 23, 570-574 (2007).

3. Effects of dietary cholesterol on tissue ceramides and oxidation products of apolipoprotein B-100 in apoE deficient mice. I. Ichi, Y. Takashima, N. Adachi, K. Nakahara, C. Kamikawa, M. Harada-Shiba, and S. Kojo, Lipids, 42, 893-900 (2007).

4. Effect of dimethyl sulphoxide on oxidative stress, activation of mitogen activated protein kinase and necrosis caused by thioacetamide in the rat liver. T. Kishioka, C. Iida, K. Fujii, R. Nagae, Y. Onishi, I. Ichi, and S. Kojo, Eur. J. Pharmacol., 564, 190-195 (2007).
5. Activation of mitogen activated protein kinase (MAPK) during carbon tetrachloride intoxication in the rat liver. C. Iida, K. Fujii, T. Kishioka, R. Nagae, Y. Onishi, I. Ichi, S. Kojo, Arch. Toxicol., 81, 489-493 (2007).
6. Inhibitory effect of dimethyl sulfoxide (DMSO) on necrosis and oxidative stress caused by D-galactosamine in the rat liver. C. Iida, K. Fujii, E. Koga, Y. Washino, I. Ichi, and S. Kojo, J. Nutr. Sci. Vitaminol., 53, 160-165 (2007).
7. Increase of ceramide in the liver and plasma after carbon tetrachloride intoxication in the rat. I. Ichi, K. Nakahara, K. Fujii, C. Iida, Y. Miyashita, and S. Kojo, J. Nutr. Sci. Vitaminol., 53, 53-56 (2007).
8. In vivo and in vitro effects of hyperglycemia on Na+-K+, Ca2+, Mg2+-dependent ATPases activity in brain synaptosomes of aging rats. T. Torlinska, A. Grochowalska, J. Kupsz, J. Skoracka, and S. Kojo, J. Physiol. Pharmacol., 57, Suppl. 7, 145-158 (2006).
9. An antioxidant role of a reagent, 2’,7’-dichlorodihydrofluorescin diacetate detecting reactive-oxygen species, blocking the induction of heme oxygenase-1 and protecting cytotoxicity. Y. Andoh, A. Mizutani, T. Ohashi, S. Kojo, T. Ishii, Y. Adachi, S. Ikehara, and S. Taketani, J. Biochem. (Tokyo), 140, 483-489 (2006).
10. Association of ceramides in human plasma with risk factors of atherosclerosis. I. Ichi, K. Nakahara, Y. Miyashita, A. Hidaka, S. Kutsukake, K. Inoue, T. Maruyama, Y. Miwa, M. Harada-Shiba, M. Tsushima, S. Kojo, and Kisei Cohort Study Group, Lipids, 41, 859-863 (2006).
11. Activation of mitogen activated protein kinase (MAPK) during D-galactosamine intoxication in the rat liver. H. Nishioka, T. Kishioka, C. Iida, K. Fujii, I. Ichi, and S. Kojo, Bioorg. Med. Chem. Lett., 16, 3019-3022 (2006).
12. The presence of oxidized phosphatidylserine on Fas-mediated apoptotic cell surface. T. Matsura, A. Togawa, M. Kai, T. Nishida, J. Nakada, Y. Ishibe, S. Kojo, Y. Yamamoto, and K. Yamada, Biochim. Biophys. Acta, 1736, 181-188 (2005).
13. Decrease in the particle size of low-density lipoprotein (LDL) by oxidation. A. Hidaka, K. Inoue, S. Kutsukake, M. Adachi, Y. Kakuta, and S. Kojo, Bioorg. Med. Chem. Lett., 15, 2781-2785 (2005).
14. A novel vitamin C analog, 2-O-(b-D-glucopyranosyl)ascorbic acid: Examination of enzymatic synthesis and biological activity.  Y. Toyoda-Ono, M. Maeda, M. Nakao, M. Yoshimura, N. Sugiura-Tomimori, H. Fukami, H. Nishioka, Y. Miyashita, and S. Kojo, J. Biosci. Bioeng. 99, 361-365 (2005).
15. Racemic D,L-asparagine causes enantiomeric excess of other coexisting racemic D,L-amino acids during recrystallization: a hypothesis accounting for the origin of L-amino acids in the biosphere. S. Kojo, H. Uchino, M. Yoshimura, and K. Tanaka, Chem. Comm., 2146 - 2147 (2004).

16. Change in liver and plasma ceramides during D-galactosamine-induced acute hepatic injury by LC-MS/MS. M. Yamaguchi, Y. Miyashita, Y. Kumagai, and S. Kojo, Bioorg. Med. Chem. Lett., 14, 4061-4064 (2004).

17. Effects of seasonings on the stability of ascorbic acid in a cooking model system. E. Kishida, T. Maeda, A. Nishimura, S. Kojo, and Y. Masuzawa, J. Nutr. Sci. Vitaminol., 50, 431-437 (2004).

18. Effect of rooibos tea (Aspalathus linearis) extract on lipid peroxidation in vitamin E deficient rats. E. Hitomi, A. Nishikiori, A. Matsumoto, S. Moriguchi, S. Kojo, S. Tokumaru, and M. Nakano, ITE Lett., 5, 64-72 (2004).

19. Mechanism for aerobic oxidation of 3,5-di-tert-butylcatechol to 3,5-di-tert-butyl-o-benzoquinone catalyzed by di-m-hydroxo-dicopper (II) complexes of peralkylated ethylenediamine ligands.  M. Kodera, K. Kawata, K. Kano, Y. Tachi, S. Itoh, and S. Kojo, Bull. Chem. Soc. Jpn, 76, 1957-1964 (2003).

20. Brain lipid hydroperoxide level increases in senescence-accelerated mice at an early age.  F. Yasui, M. Ishibashi, S. Matsugo, S. Kojo, Y. Oomura, and K. Sasaki, Neurosci. Lett., 350, 66-68 (2003).

21. Higher reactivity of apolipoprotein B-100 and a-tocopherol compared to sialic acid moiety of low density lipoprotein (LDL) in radical reaction. N. Matsukawa, Y. Nariyama, R. Hashimoto, and S. Kojo, Bioorg. Med. Chem., 11, 4009-4013 (2003).

22. Change in the concentration of vitamins C and E in rat tissues by paraquat administration.  K. Ikeda, Y. Kumagai, Y. Nagano, N. Matsuzawa, and S. Kojo, Biosci. Biotech. Biochem., 67, 1130-1131 (2003).

23. Evaluation of oxidative stress during apoptosis and necrosis caused by D-galactosamine in rat liver. F. Sun, E. Hamagawa, C. Tsutsui, N. Sakaguchi, Y. Kakuta, S. Tokumaru, and S. Kojo, Biochem. Pharmacol., 65, 101-107 (2003).

24. Effects of chronic acetyl-L-carnitine treatment on brain lipid hydroperoxide level and passive avoidance learning in senescence-accelerated mice. F. Yasui, S. Matsugo, M. Ishibashi, T. Kajita, Y. Ezashi, Y. Oomura, S. Kojo, and K. Sasaki, Neurosci. Lett., 334, 177-180 (2002).

25. Evaluation of apolipoprotein B-100 fragmentation and cross-link in the serum as an index of atherosclerosis. R. Hashimoto, N. Matsukawa, Y. Nariyama, Y. Ogiri, E. Hamagawa, K. Tanaka, Y. Usui, S. Nakano, T. Maruyama, S. Kyotani, M. Tsushima, and S. Kojo, Biochim. Biophys. Acta, 1584, 123-128 (2002).

26. Induction of apoptosis in HL-60 cells by photochemically generated hydroxyl radicals.  S. Haruna, R. Kuroi, K. Kajiwara, R. Hashimoto, S. Matsugo, S. Tokumaru, and S. Kojo, Bioorg. Med. Chem. Lett., 12, 675-676 (2002).

27. Very low vitamin C activity of orally administered L-dehydroascorbic acid. Y. Ogiri, F. Sun, S. Hayami, A. Fujimura, K. Yamamoto, M. Yaita, and S. Kojo, J. Agric. Food Chem., 50, 227-229 (2002).

28. Rapid oxidation of dichlorofluorescin with heme and hemoproteins: Formation of the fluorescein is independent of the generation of reactive oxygen species. T. Ohashi, A. Mizutani, A. Murakami, S. Kojo, T. Ishii, and S. Taketani, FEBS Lett., 511, 21-27 (2002).

29. Role of serum components in the binding and phagocytosis of oxidatively damaged erythrocytes by autologous mouse macrophages. K. Tanaka, Y. Usui, and S. Kojo, Cell. Mol. Life Sci., 58, 1727-1733 (2001).

30. Enantioselective crystallization of D,L-amino acids induced by spontaneous asymmetric resolution of D,L-asparagine. S. Kojo, and K. Tanaka, Chem. Commun., 1980-1981 (2001).

31.  Increase of ceramides and its inhibition by catalase during chemically induced apoptosis of HL-60 cells determined by electrospray ionization tandem mass spectrometry. Y. Yamada, K. Kajiwara, M. Yano, E. Kishida, Y. Masuzawa, and S. Kojo, Biochim. Biophys. Acta, 1532, 115-120 (2001).

32. Hydrogen peroxide and hydroxyl radical involvement in the activation of caspase-3 in chemically induced apoptosis of HL-60 cells. K. Kajiwara, K. Ikeda, R. Kuroi, R. Hashimoto, S. Tokumaru, and S. Kojo, Cell. Mol. Life Sci., 58, 485-491 (2001).

33. Protective effect of fluvastatin on degradation of apolipoprotein B by radical reaction in human plasma. S. Aoki, K. Ikeda, M. Yamamura, and S. Kojo, Biol. Pharm. Bull., 24, 123-126 (2001).

34.  Evaluation of oxidative stress during apoptosis and necrosis caused by carbon tetrachloride in rat liver.  F. Sun, C. Tsutsui, E. Hamagawa, Y. Ono, Y. Ogiri, and S. Kojo, Biochim. Biophys. Acta, 1535, 186-191 (2001).

35.  Unusually high reactivity of apolipoprotein B-100 among proteins to radical reactions induced in human plasma.  R. Hashimoto, S. Narita, Y. Yamada, K. Tanaka, and S. Kojo, Biochim. Biophys. Acta, 1483, 236-240 (2000).

36.  Evaluation of oxidative stress based on lipid hydroperoxide, vitamin C and vitamin E during apoptosis and necrosis caused by thioacetamide in rat liver.  F. Sun, S. Hayami, Y. Ogiri, S. Haruna, K. Tanaka, Y. Yamada, S. Tokumaru, and S. Kojo, Biochim. Biophys. Acta, 1500, 181-185 (2000).

37. Age-dependent changes in lipid peroxide levels in various organs in senescence-accelerated mouse. S. Matsugo, T. Kitagawa, S. Minami, Y. Esashi, Y. Oomura, K. Sasaki, S. Tokumaru, S. Kojo, and K. Matsushima, Neurosci. Lett., 278, 105-108  (2000).

38.  Docosahexaenoic acid and vitamin E can reduce human monocytic U937 cell apoptosis induced by tumor necrosis factor. M. Yano, E. Kishida, M. Iwasaki, S. Kojo ,and Y. Masuzawa, J. Nutr., 130, 1095-1101 (2000).

39.  In vivo antioxidant activity of propolis evaluated by the interaction with vitamin C and E, and the level of lipid hydroperoxides in rats. F. Sun, S. Hayami, S. Haruna, Y. Ogiri, K. Tanaka, Y. Yamada, K. Ikeda, H. Yamada, H. Sugimoto, N. Kawai, and S. Kojo, J. Agric. Food Chem., 48, 1462-1465 (2000).

40.  Change in caspase-3-like protease in the liver and plasma during rat liver regeneration following partial hepatectomy. S. Hayami, M. Yaita, Y. Ogiri, F. Sun, R. Nakata, and S. Kojo, Biochem. Pharmacol., 60, 1883-1886 (2000).

41.  Inhibition of radical reaction of apolipoprotein B-100 and a-tocopherol in human plasma by green tea catechins. R. Hashimoto, M. Yaita, K. Tanaka, Y. Hara, and S. Kojo, J. Agric. Food Chem., 48, 6380-6383 (2000).

42.  Change in tissue concentrations of lipid hydroperoxides, vitamin C and vitamin E in rats with streptozotocin-induced diabetes.  F. Sun, K. Iwaguchi, R. Shudo, Y. Nagaki, K. Tanaka, K. Ikeda, S. Tokumaru, and S. Kojo, Clin. Sci., 96, 185-190 (1999).

43.  Facile degradation of apolipoprotein B by radical reactions and the presence of cleaved proteins in serum.  K. Tanaka, H. Iguchi, S. Taketani, R. Nakata, S. Tokumaru, T. Sugimoto, and S. Kojo, J. Biochem. (Tokyo), 125, 173-176 (1999).

44. Involvement of hydrogen peroxide and hydroxyl radical in chemically induced apoptosis of HL-60 cells. K. Ikeda, K. Kajiwara, E. Tanabe, S. Tokumaru, E. Kishida, Y. Masuzawa, and S. Kojo, Biochem. Pharmacol., 57, 1361-1365 (1999).

45.  Comparison of reactivity among carbohydrate moieties of transferrin to a radical reaction.  K. Tanaka, Y. Yamada, S. Narita, R. Hashimoto, and S. Kojo, Res. Commun. Biochem. Cell Mol. Biol., 3, 63-68 (1999).

46.  Increase of caspase-3 activity in rat liver and plasma by thioacetamide. S. Hayami, K. Ikeda, F. Sun, K. Tanaka, and S. Kojo, Biochem. Pharmacol., 58, 1941-1943 (1999).

47.  Increase of lipid hydroperoxides in liver mitochondria and inhibition of cytochrome oxidase by carbon tetrachloride intoxication in rats.  K. Ikeda, M. Toda, K. Tanaka, S. Tokumaru, and S. Kojo, Free Radic. Res., 28, 403-410 (1998).

48.  Increase of lipid hydroperoxides in the rat liver and kidney after ferric nitrilotriacetate administration.  K. Ikeda, F. Sun, K. Tanaka, S. Tokumaru, and S. Kojo, Biosci. Biotechnol. Biochem., 62, 1438-1439 (1998).

49.  Change in the level of sialic acid in plasma, brain and liver of inherently scorbutic rats during vitamins C and E deficiencies.  K. Tanaka, S. Tokumaru, and S. Kojo, Biosci. Biotechnol. Biochem., 62, 1592-1593 (1998).

50.  Degradation of transferrin and albumin by radical reactions in human plasma evaluated by immunoblot.  Y. Yamada, E. Okamoto, K. Tanaka, and S. Kojo, Biochem. Mol. Biol. Int., 46, 733-738 (1998).

51.  Rat alveolar macrophages produce O2- and NO in the culture with JFM standard fibers.  H. Iguchi and S. Kojo, in "Excerpta Medica International Congress Series 1153: Advances in the Prevention of Occupational Respiratory Diseases", pp. 615-620, K. Chiyotani, Y. Hosoda and Y. Aizawa, Eds, Elsevier Science, Amsterdam, The Netherlands (1998).

52.  Increase of lipid hydroperoxides in tissues of vitamin E-deficient rats.  S. Tokumaru, R. Ogino, A. Shiromoto, H. Iguchi, and S. Kojo, Free Radic. Res., 26, 169-174 (1997).

53.  Possible involvement of radical reactions in desialylation of LDL.  K. Tanaka, S. Tokumaru, and S. Kojo, FEBS Lett., 413, 202-204 (1997).

54.  Interactions between vitamin C and vitamin E are observed in tissues of inherently scorbutic rats.  K. Tanaka, T. Hashimoto, S. Tokumaru, H. Iguchi, and S. Kojo,

     J. Nutr., 127, 2060-2064 (1997).

55.  Change of the lipid hydroperoxide level in mouse organs on ageing.  S. Tokumaru, H. Iguchi, and S. Kojo, Mech. Ageing Dev., 86, 67-74 (1996).

56.  NO synthase activity in the lung and NO synthesis in alveolar macrophages of rats increased on exposure to asbestos. H. Iguchi, S. Kojo, and M. Ikeda, J. Appl. Toxicol., 16, 309-315 (1996).

57.  Change in the level of vitamin C and lipid peroxidation in tissues of the inherently scorbutic rat during ascorbate deficiency.  S. Tokumaru, S. Takeshita, R. Nakata, I. Tsukamoto, and S. Kojo, J. Agric. Food Chem., 44, 2748-2753 (1996).

58.  Specific and sensitive determination of lipid hydroperoxides with chemical derivatization into 1-naphthyldiphenylphosphine oxide and high-performance liquid chromatography. S. Tokumaru, I. Tsukamoto, H. Iguchi, and S. Kojo, Anal. Chim. Acta, 307, 97-102 (1995).

59.  Re-evaluation of malondialdehyde and thiobarbituric acid-reactive substances as indices of autoxidation based on the oxygen consumption.  E. Kishida, A. Kamura, S. Tokumaru, M. Oribe, H. Iguchi, and S. Kojo, J. Agric. Food Chem., 41, 1-4 (1993).

60.  Lipid peroxidation and disintegration of the cell membrane structure in cultures of rat lung fibroblasts treated with asbestos. H. Iguchi, S. Kojo, and M. Ikeda, J. Appl. Toxicol., 13, 269-275 (1993).

61.  Effect of ageing on rat liver regeneration after partial hepatectomy.  I. Tsukamoto, R.   Nakata, and S. Kojo,  Biochem. Mol. Biol. Int., 30, 773-778 (1993).

62.  Comparison of the formation of malondialdehyde and thiobarbituric acid-reactive substances from autoxidized fatty acids based on oxygen consumption. E. Kishida, S. Tokumaru, Y. Ishitani, M. Yamamoto, M. Oribe, H. Iguchi, and S. Kojo, J. Agric. Food Chem., 41, 1598-1600 (1993).

63.  The effects of methotrexate on rat liver regeneration after partial hepatectomy.  I. Tsukamoto, and S. Kojo, Clin. Sci., 82, 181-184 (1992).

64.  Peroxyoxalate chemiluminescent assay in aqueous solution with g-cyclodextrin. S. Kojo, S. Tokumaru, E. Kishida, and I. Tsukamoto, Clin. Chem., 38, 788-788 (1992).

65.  Specific determination of ascorbic acid with chemical derivatization and high-performance liquid chromatography.  E. Kishida, Y. Nishimoto, and S. Kojo, Anal. Chem., 64, 1505-1507 (1992).

66.  Effect of endotoxin on rat liver regeneration after partial hepatectomy.  I. Tsukamoto, R. Nakat,a and S. Kojo, Biochem. Int., 27, 1047-1050 (1992).

67.  Early decreases in pulmonary, hepatic and renal glutathione levels in response to cadmium instillation into rat trachea.  H. Iguchi, S. Kojo, and M. Ikeda, J. Appl. Toxicol., 11, 211-217 (1991).

68.  The effects of fluorouracil on thymidylate synthase and thymidine kinase in regenerating rat liver after partial hepatectomy.  I. Tsukamoto, and S. Kojo, Biochim. Biophys. Acta, 1074, 52-55 (1991).

69.  Effect of interferone inducer, polyriboinosinic-polyribocytidylic acid on rat liver regeneration following partial hepatectomy.  I. Tsukamoto, and S. Kojo, Biochem. Int., 23, 619-624 (1991).

70.  Effect of the remaining ischemic liver lobes on DNA synthesis in rat liver regeneration following 70% functional hepatectomy.  I. Tsukamoto, and S. Kojo, Biochem. Int., 23, 923-926 (1991).

71.  Purification and charaterization of thymidine kinase from regenerating rat liver.  I. Tsukamoto, Y. Taniguchi, M. Miyoshi, and S. Kojo, Biochim. Biophys. Acta, 1079, 348-352 (1991).

72. Relationship among malondialdehyde, TBA-reactive substances and tocopherols in the oxidation of soybean oil. E. Kishida, M. Oribe, and S. Kojo, J. Home Econ. Jpn., 42, 219-222 (1991).

73.  The sex difference in the regulation of liver regeneration after partial hepatectomy in the rat.  I. Tsukamoto, and S. Kojo, Biochim. Biophys. Acta, 1033, 287-290 (1990).

74.  Effect of a-blockade on liver regeneration after carbon tetrachloride intoxication in the rat.  Y. Ochi, Y. Yumori, A. Morioka, K. Miura, I. Tsukamoto, and S. Kojo, Biochem. Pharmacol., 39, 2065-2066 (1990).

75.  Determination of malondialdehyde with chemical derivatization into the pyrimidine compound and HPLC. E. Kishida, M. Oribe, K. Mochizuki, S. Kojo, and H. Iguchi, Biochim. Biophys. Acta, 1045, 187-188 (1990).

76.  Effect of cycloheximide and actinomycin D on rat liver regeneration after partial hepatectomy.  I. Tsukamoto, and S. Kojo, J. Nutr. Sci. Vitaminol., 36, 357-363 (1990).

77.  Relationship among malondialdehyde, TBA-reactive substances and tocopherols in the oxidation of rapeseed oil.  E. Kishida, M. Oribe, and S. Kojo, J. Nutr. Sci. Vitaminol., 36, 619-623 (1990).

78.  Erhalt der Konfiguration bei der nicht-Darwinistischen Evolution von Cytochrom c. S. Kojo, K. Fukunishi, and I. Tsukamoto, Angew. Chem., 101, 64-65 (1989). 

      Retention of configuration in the non-Darwinian evolution of cytochrome c.

    Angew. Chem. Int. Ed. Engl., 28, 71-72 (1989).

79.  Possible generation of hydrogen peroxide and lipid peroxidation of erythrocyte membrane by asbestos: cytotoxic mechanism of asbestos. H. Iguchi, and S. Kojo, Biochem. Int., 18, 981-990 (1989).

80.  Effect of glucocorticoid on liver regeneration after partial hepatectomy in the rat.  I. Tsukamoto, and S. Kojo, Gut,  30, 387-390 (1989).

81.  Effect of colchicine and vincristine on DNA synthesis in regenerating rat liver. I. Tsukamoto, and S. Kojo,  Biochim. Biophys. Acta, 1009, 191-193 (1989).

82. A new immunoblotting assay for thymidylate synthetase and its application to the regulation of enzyme activity in regenerating rat liver. I. Tsukamoto, R. Nakata, M. Miyoshi, S. Taketani, and S. Kojo,  Biochim. Biophys. Acta, 964, 254-259 (1988).

83.  Photoacetylation of 2-substituted adamantanes. Stereochemistry and substituent effects.  K. Fukunishi, A. Kohno, and S. Kojo, J. Org. Chem., 53, 4369-4374 (1988).

84.  Effect of thyroparathyroidectomy on the activities of thymidylate synthetase and thymidine kinase during liver regeneration after partial hepatectomy. R. Nakata, I. Tsukamoto, M. Miyoshi, and S. Kojo, Clin. Sci., 72, 455-461 (1987).

85.  Purification and characterization of thymidylate synthetase from rat regenerating liver.  R. Nakata, I. Tsukamoto, M. Miyoshi, and S. Kojo, Biochim. Biophys. Acta, 924, 297-302 (1987).

86. Effect of phorbol ester on rat liver regeneration. I. Tsukamoto, and S. Kojo, Biochem. Pharmacol., 36, 2871-2872 (1987).

87.  Electron transfer reaction from dimethylanilines to cation radical of magnesium octaethylporphyrin.  S. Kojo, K. Morimitsu, and I. Tabushi,  Chem. Lett., 2095-2096 (1987).

88.  Effect of calcium channel blockers and trifluoperazine on rat liver regeneration.  I. Tsukamoto, and S. Kojo, Eur. J. Pharmacol., 159-162 (1987).

89.  One evidence supporting that thymidylate synthetase and thymidine kinase are the rate-determining enzymes of DNA synthesis in regenerating rat liver.  I. Tsukamoto, and S. Kojo, Chem. Lett., 2313-2316 (1987).

90.  Liver regeneration in streptozotocin-diabetic rats.  R. Nakata, I. Tsukamoto, M. Miyoshi, and S. Kojo,  Biochem. Pharmacol., 35, 865-867 (1986).

91.  Liver regeneration after carbon tetrachloride intoxication in the rat.  R. Nakata, I. Tsukamoto, and S. Kojo,  Biochem. Pharmacol., 34, 586-588 (1985).

92.  Cyclodextrin-promoted free-radical dediazoniation of benzenediazonium ions. K. Fukunishi, J. Hira, H. Yamanaka, M. Nomura, and S. Kojo, J. Chem. Soc. Perkin I, 991-995 (1985).

93.  a-Adrenergic regulation of the activity of thymidylate synthetase and thymidine kinase during liver regeneration after partial hepatectomy.  R. Nakata, I. Tsukamoto, M. Nanme, S. Makino, M. Miyoshi, and S. Kojo, Eur. J. Pharmacol., 114, 355-360 (1985).

94.  Hydroxylation of benzenes involving the NIH shift utilizing electronic model compound for monooxygenases.  S. Kojo, and K. Fukunishi, Chem. Lett., 1707-1710 (1983).

95.  Exclusive free radical dediazoniation of benzenediazonium ions by b-cyclodextrin. K. Fukunishi, H. Kazumura, H. Yamanaka, M. Nomura, and S. Kojo, J. Chem. Soc. Chem. Commun., 799-800 (1982).

96.  Chemistry of 2-substituted pyrimidines. Studies toward the synthesis of the pyrimidine moiety of bleomycin.  W. K. Hagman, F. Z. Basha, M. Hashimoto, R. B. Frye, S. Kojo, and S. M. Hecht, J. Org. Chem., 46, 1413-1423 (1981).

97.  Mechanism of novel synthesis of haemin c from protohaemin and L-cysteine.  Markownikoff-type radical addition reaction. S. Kojo, and S. Sano, J. Chem. Soc. Perkin I, 2864-2870 (1981).

98.  Degradation of horse heart cytochrome c to a single diastereoisomeric porphyrin c.  S. Kojo, S. Sano, and K. Fukunishi, J. Chem. Soc. Chem. Commun., 1223-1224 (1981).

99.  Mechanism of photoacetylation of substituted adamantanes. I. Tabushi, S. Kojo, and K. Fukunishi, J. Org. Chem., 43, 2370-2374 (1978).

100.  Synthesis of hemin c from hemin. S. Kojo and S. Sano, J. Chem. Soc. Chem. Commun., 249-250 (1977).

101. Physical-organic approach to the reactivities of species bearing unpaired electron.

       S. Kojo, PhD Thesis, Kyushu University (1975).

102. Intermediacy of hematoporphyrin-Mn(III) cation radical in the decay of hemato-porphyrin-Mn(IV) in acidic media.  I. Tabushi, and S. Kojo, Tetrahedron Lett., 305-308 (1975).

103. Selective functionalization of unactivated methine positions. 4-Acetyldiamantane. I. Tabushi, S. Kojo, P. v. R. Schleyer, and T. M. Gund, J. Chem. Soc. Chem. Commun., 591 (1974).

104.  Photochemistry of N2O5 with adamantanes. I. Tabushi, and S. Kojo, Chem. Lett., 1431-1434 (1974).

105. Complex pH-rate profile of hematoporphyrin-Mn (IV) decay.  I. Tabushi, and S. Kojo, Tetrahedron Lett., 1577-1580 (1974).

106. Photoacetylation of substituted adamantanes.  Exclusive bridge-head substitution and a large r * value. I. Tabushi, S. Kojo, and Z. Yoshida, Tetrahedron Lett., 2329-2332 (1973).

107.  Free-radical halogenation of adamantane. Selectivity and relative lifetime of 1- and 2-adamantyl radicals.  I. Tabushi, Y. Aoyama, S. Kojo, J. Hamuro, and Z. Yoshida, J. Am. Chem. Soc., 94, 1177-1183 (1972).



Laboratory of Food Safety and Toxicology Home Page
Food Science and Nutrition Home Page
Faculty of Human Life and Environment Home Page

Updated 2007.01.15